Mooring Day!

Flotation going in, with yours truly in the background.
What’s that entering my environment??

A few days ago we deployed three moorings in our study area. These complicated contraptions  hold instruments in place in the ocean’s water column, strung on a line between a bottom anchor weight and surface (or subsurface) flotation. Big excitement for Team Phytoplankton because the instruments (if they work AND survive the storms) will provide a continuous stream of data on the ocean’s temperature, salinity, subsurface light environment, chlorophyll and nutrient content, and many other wondrous things. Although they will only be sampling one point in space, this continuous time record will allow us to ask all kinds of questions about the timing of phytoplankton blooms and their relationship to ocean and atmosphere conditions. As you’ll see below, many other questions can be asked as well, by other members of our science team and the larger community.

Sikuliaq bosun (overseer of deck operations) Paul, overseeing deployment of an AZFP ( acoustic device to measure the presence of zooplankton and small fish) on one of the mooring lines.

It’s one thing to see theoretical diagrams of mooring design on paper and another to see these enormous and complex arrangements of gear being deployed off the stern of Sikuliaq. We first spent some hours surveying the bottom in the deployment area, using the ship’s multi-beam seismic survey instruments to fine-tune the deployment location. (The moorings were designed for a depth of 230 meters.) The flotation goes in first, and then the line is strung out behind the ship as it slowly steams along. Gear is added using shackles, pear links, winches, etc. to take tension off of the main line and allow the instruments to be attached. Finally the anchor weights (railroad wheels!) are lowered over the side, and with the whole 230 meter-long mooring now streaming out behind the ship, they are released using a snatch block. As the weights descend they briefly pull the surface flotation under and it is an adrenaline-charged moment to wait for that flotation to pop up again – did we really calculate that line length properly and find the right bottom depth for deployment???

Anchor weight, made of railroad wheels, being hoisted off the deck.
Anchor weight at sea surface, ready to be released using the snatch block – just yank the green line and cross your fingers!
Mooring lead scientist Seth Danielson and mooring tech Pete Shipton programming the wire walker before deployment.

Three moorings were deployed. One is a guard mooring with only a few low-cost instruments on it. The second has a ‘wire walker’ instrument package that travels up and down the mooring line twice per day measuring temperature, salinity and other properties from near the bottom to near the surface on each excursion. The third is filled with instrumentation in addition to the ‘standard’ oceanographic sensors, including ADCPs*; both passive acoustics (listening for marine mammals) and active acoustics (using sound waves to identify large zooplankton and fish); a sediment trap that collects sinking material and funnels it into a preservative-filled cup, rotating to fill a new cup every two weeks; and a particle imaging system. It is truly impressive to watch an expensive instrument the size of, say, a large fire hydrant being almost literally tossed into the ocean many miles (about 75) from shore. The skill of our mooring technician Pete, the ship’s bosun Paul, and the other crew involved in this all-day operation was incredibly impressive and the whole operation went flawlessly.

For the science nerds among you, some of the mooring data are being transmitted to shore and can be viewed on the Alaska Ocean Observing System (AOOS) web site. Check it out: we are the GEO moorings in the Gulf of Alaska section.

Sediment trap being attached to mooring line. Note the tall grey collector and funnel, which will sequentially fill the clear bottles on the lower part of the apparatus.
Surface float with meteorological instruments.
Big ocean, small mooring. We hope to see you in Spring 2020!

*Acronym of the day: ADCP = acoustic doppler current profiler

Life in a Bubble

Post by UAF graduate student and honorary Team Phytoplankton member Annie Kandel

I’m not an official member of Team Plankton, but my advisor at University of Alaska Fairbanks, Dr. Ana Aguilar-Islas, works closely with Suzanne on these cruises. I’m also sharing a room with Kerri (Team Phytoplankton Super-Tech!) so that has to count for something. We’ve had a lot of good bonding moments, like the couple of times she nearly witnessed my death as I tried to climb down from the top bunk.

Annie showing her enthusiasm for the quartz tubes used to study iron photochemistry. They will be filled with seawater and incubated on deck.

When I’m not falling out of bed, I spend a decent amount time with Ana and our other team member, Carrie, working in a plastic bubble we set up in Sikuliaq’s analytical lab. Most of our work on the LTER cruises is sampling for trace metals, and trace metal clean techniques can be kind of intense. Amazing care has to be taken to prevent contamination of seawater samples containing minute quantities of iron, on a ship that is literally made out of iron. Our bubble took the whole first day to set up and included building a frame out of line and wood, setting up a rack for our trace metal clean Niskin bottles (which spend as little time outside the bubble as possible), hanging HEPA* units and a lot of plastic, and then cutting windows in the plastic so that it doesn’t look quite so suspicious. Also, so that we can see the clock.

“The Bubble”, for sampling and processing trace metal samples without contamination, built by the trace metal sampling team and ship’s crew in Sikuliaq’s analytical lab.

While we’re taking or processing samples, we wear sleeves as well as gloves to protect the samples from ourselves, and we also wear different shoes in the bubble. Since we’re working with naturally occurring metals such as iron and aluminum it’s really important to make sure we don’t get any hair, skin, or dust in the bottles. Iron is an important trace metal in the ocean, as it’s required by various enzymes for biological processes but is generally found in very small (nanomolar) concentrations. There are different forms of iron in the ocean and the fraction referred to as “dissolved” is the pool that is biologically available. Dissolved iron only constitutes about 1% or less of the total iron that is present in seawater, and in many areas of the ocean iron acts as a limiting nutrient for phytoplankton growth.

These waters are called HNLC, which stands for high nutrient/low chlorophyll, and it means that the chlorophyll levels are lower than would normally be expected given the amount of available macronutrients, e.g. nitrate and phosphate. The discrepancy is thought to be due to iron limitation. To investigate this, we’re performing an iron addition experiment where we collect HNLC water and add iron both naturally, by mixing it with water from the Copper River plume, as well as artificially, by spiking samples with an iron(III) chloride solution. The experiment was set up a couple of days ago after many hours of searching for HNLC water. We are taking samples daily to see how the plankton community composition and physiology changes under these conditions.

The last time Ana and Suzanne performed this experiment their bottles had been sitting in acid for 10 years, a cleaning procedure that is excessive even by trace metal standards. This time around I was in charge of cleaning them and only had about two months, so keep your fingers crossed!

*Acronym of the day: HEPA = High Efficiency Particulate Air. These filter units remove dust and other particles that could carry iron into the seawater samples being processed.

Why do Science? The view from the high seas

Post by WWU graduate student Clay Mazur

The other day I was asked by Kat, our NOAA Teacher-At-Sea for this cruise, what I want members of the general public to take away from my work studying iron limitation of phytoplankton. Though I can provide her a superficial answer to my research question immediately, the motivations for my work go much deeper than answering “How does a micronutrient affect phytoplankton growth?”

There are two main levels at which I want to answer Kat’s question:

1. Proximal: Though phytoplankton are microscopic, they have macroscopic impacts.

2. Philosophical: Why bother in the quest for such knowledge?

Level 1: The Macroscopic Impacts of a Microscopic Organism 

A single cell of the phytoplankton species Emiliania huxleyi. The calcium carbonate plates surrounding the cell are vulnerable to dissolution by excess CO2 in seawater. The cell, only 1/200th of a millimeter in diameter, was imaged with a scanning electron microscope (courtesy B. Olson and B. Love, WWU marine scientists)

Both human societies and phytoplankton communities are impacted by global climate change. Globally, humans are realizing the need to combat carbon emissions and mediate the effects of increasing global temperatures. Consequences of global climate change for us include mass emigration as sea levels rise, and increased frequency of extreme weather events (e.g. droughts, wildfires). As a result, humans are racing to bridge political divides between countries, develop sustainable energy, and manage natural disaster response. Phytoplankton, too, must respond to global climate change. As sea surface temperatures rise, phytoplankton will have to adapt. Excess CO2 that enters seawater from the atmosphere – increasing as humans burn ever more fossil fuel – can remove the precious materials some plankton use to make their “shells” and take away their protection. Dissolved CO2 can also alter the ability of micrograzers to swim and find food! Melting glaciers are a double-edged sword. Glacial flour (finely ground rock) in freshwater runoff brings in vital nutrients (including iron) through the Copper River Plume and phytoplankton love their iron! But freshwater also works to trap phytoplankton in the surface layers. When all the nutrients are used up and you’re a phytoplankton baking in the heat of the sun, being trapped at the surface is super stressful! As global climate change accelerates in the polar regions, phytoplankton in the Northern Gulf of Alaska are in an evolutionary race against time to develop traits that make them resilient to their ever-changing environment. Phytoplankton crossing the finish line of this race is imperative for us humans, since phytoplankton help to mediate climate change by soaking up atmospheric CO2 during photosynthesis to produce ~ 50 % of the oxygen we breathe!

Kittiwakes surrounding a shipwreck on Middleton Island in May 2019. The ship’s hull is now a seabird nesting habitat.

Phytoplankton also form the base of a complex oceanic food web. The fresh salmon in the fish markets of Pike’s Place (Seattle, WA), the gigantic gulp of a humpback whale in Prince William Sound (AK) and even entire colonies of kittiwakes on Middleton Island (AK) are dependent on large numbers of phytoplankton. When phytoplankton are iron limited, they cannot grow or multiply (via mitosis). In a process called bottom-up regulation, the absence of phytoplankton reduces the growth of animals who eat phytoplankton, the animals who eat those animals, and so on up the entire food chain. To illustrate this point, let us consider “The Blob”, an area of elevated sea surface temperature in the Gulf of Alaska in 2015-16. “The Blob” limited phytoplankton growth, thereby reducing the abundance of small, fatty fishes further up the food web. As a result, the population of kittiwakes on Middleton Island crashed as the birds could not find enough fish to provide them the nutrients and energy to reproduce successfully. In this way, the kittiwake population decline was directly attributable to a lack of phytoplankton production.

Pink salmon, an important commercial fishery in the northern Gulf of Alaska

Not only are phytoplankton ecologically important, they are commercially important. For consumers who love to fish (and for the huge commercial fisheries in the Northern Gulf of Alaska), the base of the food web should be of particular interest, as it is the harbinger of change. Fisheries managers may use models of phytoplankton growth to monitor fish stocks and establish fisheries quotas. If sporadic input of iron from dust storms, glacial runoff, or upwelling stimulate phytoplankton to grow, fish stocks may also increase with the newfound food source. Because phytoplankton are inextricably linked to fish, whales, and seabirds, in years where nutrients are plentiful, you may well see more fish on kitchen tables across the U.S. and Native Alaskans may be able to harvest more seabird eggs.

Level 2: The Nature of Science

As a supporter of place-based and experiential learning, I view myself as a student with a dual scientist-educator role. To succeed in these roles, I have to be able to combine reasoning with communication and explore questions like “How does science relate to society?” and “How do we foster scientific literacy?” What better way to think about these questions than embarking on a three-week cruise to the Pacific Subarctic?! Not only am I working with amazing Principal Investigators in an immersive research experience, I am able to collect data and think of creative ways to communicate my findings. These data can be used to build educational curricula in an effort to merge the classroom with the Baltic room (where the CTD is deployed). But what’s the point of collecting data and sharing it?

Science is “a collaborative enterprise, spanning the generations” (Carl Sagan) and is “the poetry of reality” (Richard Dawkins). The goal of communicating my results in a way that touches the lives of students is two-fold. One aim is to allow them to appreciate the philosophy of science- that it is iterative, self-correcting, and built upon measurable phenomena. It is the best way that we “know” something. The other aim is to allow students to engage in scientific discourse and build quantitative reasoning skills. As the renowned astrophysicist, Neil DeGrasse Tyson has said, “when you’re scientifically literate the world looks very different to you and that understanding empowers you.” Using phytoplankton to model the scientific process allows students to enter into the scientific enterprise in low-stakes experiments, to question how human actions influence ecosystems, and to realize the role science plays in society. Ultimately, I want students to use my data to learn the scientific process and build confidence to face the claims espoused by the U.S. government and seen on Facebook with a healthy amount of skepticism and an innate curiosity to search for the truth.

Do you Scuba Dive? No, we CTD!

One of the first questions I often get, once people hear that I’m an oceanographer, is, “Do you scuba* dive?” I actually used to, way back in the day when I worked on corals as an undergraduate, but diving turns out not to be part of most oceanographic expeditions. So how do we get our samples out of the ocean?

CTD being recovered through the side bay of Sikuliaq. Instruments are at bottom of package, with rosette of long, cylindrical water sampling bottles above.

Allow me to introduce the CTD, mentioned in previous posts but not really explained. The CTD (short for Conductivity, Temperature and Depth) is an instrument package surrounded by water collection bottles (called ‘Niskin’ bottles) that we put over the side of the ship multiple times per day to collect data about the water ‘column’ as well as water samples containing the dozens of things we’d like to measure directly: suspended particles like the ones coming in with the glacier-fed rivers, dissolved chemicals including the nutrients that sustain plankton growth, and the smallest members of the planktonic community including the myriad of species studied by Team Phytoplankton.

CTD packages come in all sizes and we have an impressively large one on Sikuliaq. It’s picked up off the deck by a winch and then boomed out off the side of the ship through a sort of garage door, making it possible to deploy even in fairly rough weather (which we haven’t had yet – it’s been Lake Alaska out here so far this cruise). On the smaller ships we sometimes use, the CTD deployment and recovery is much more ‘hands on’ and can get very exciting in rough seas.

Got monitors? The CTD output screen is at lower left.

One of the coolest parts of the CTD is the real-time data connection to computers on board, via a conducting cable that also acts as the tether to the ship (you don’t want to kink or break this one. . . tedious to repair and very expensive to replace). As the package of instruments and bottles descends through the water, data are fed up the cable, collected by a computer, and plotted for us to see. We can get information in real time about depths where the temperature, salinity and dissolved oxygen change sharply (marking potential changes in habitat for marine organisms). We can see chlorophyll fluorescence maxima, marking layers rich in phytoplankton. Other sensors we have measure nitrate (an important source of N for phytoplankton) and light (of obvious importance for photosynthesis!). We also have some very high-tech instruments that capture images of particles at different depths.

As the CTD is hauled up again, we stop it at depths of interest and ‘fire’ one of the sampling bottles by clicking a box on the computer screen. This sends an electrical impulse down the conducting cable, releasing the bottle’s top and bottom closure lids from a harness and allowing them to snap close and trap a water sample.

and WWU graduate student Clay
M. collect water samples from the Niskin bottles after the cast.
Let the sampling begin! Undergraduate (REU) student Delphina W-P

When the whole package comes on board we end up with an array of water samples collected from different depths. Everyone swarms in to drain them into a motley collection of bottles and bags. There are so many different properties being sampled on Sikuliaq that we have to make a ‘water budget’ allocating the 12 liters in each Niskin to each person who needs some, and even specifying the sampling order in some cases.

Our deepest casts on this cruise will be to 2,000 meters (more than 1 mile) deep, and will take several hours. You can see that a scuba diver would have to be mighty well equipped and extremely pressure-resistant to do the job of the CTD!

CTD data from the Copper River plume, shown as a ‘vertical profile’ with depth on the vertical axis and several water properties shown. Note the very fresh lens on the surface (blue line), the strong decrease in temperature with depth (red line) and the two subsurface chlorophyll maxima (green line).

*Acronym of the day: SCUBA = Self-Contained Underwater Breathing Apparatus

Wait, Who’s Team Plankton? And what DO you DO?

Posted by Kerri Fredrickson, Research Technician

It was fun to read Delphina’s post yesterday, and look through the eyes of someone experiencing their first research cruise. Her post made me reflect on my past research cruises – although I am starting to lose count! How many times has it been…

I don’t know who to credit to for this meme, but it is pretty spot on.

It’s hard to describe what my job at sea is like, but someone sent me this meme that sums it up pretty well. Multitasking? You bet. We have a lot of wheels spinning in our lab. Team Plankton (aka the Strom Lab) is led by our fearless leader, Suzanne. She kindly gives me a lot of credit for holding things together, but her knowledge, leadership skills and sea-going abilities are impressive. Suzanne is one of the NGA LTER principal investigators (PIs). I am Suzanne’s research technician on the LTER project, and I conducted my graduate work in the Gulf of Alaska with her 15 (!) years ago.  You’ve met our two graduate students, Clay and Hana. Both are off to a roaring start with their thesis projects. Rounding out our team is Delphina, who set up her first experiment just a few hours ago! Combining two new thesis projects, a REU project, intensive plume study sampling and experimentation, plus our “standard” NGA LTER sampling – we have our hands full. When the CTD hits the deck, there are a lot of things happening at once.

Team Plankton: Delphina (REU), Suzanne (PI), Clay (graduate student), Kerri (tech), Hana (graduate student) aboard the R/V Sikuliaq.

Problem Solving. As Delphina mentioned yesterday, there is no going to the store or supply closet. I pack doubles, triples, spares, extras of everything – while trying to anticipate the possible needs of all our research projects. Still, equipment malfunctions (the physical oceanographers are heroically trying to repair one major piece of sampling equipment as I type this), the cryovials are nowhere to be found, fragile glassware breaks…it all happens. Time passes quickly and there’s not a lot of room for error. This bring us to:

Requires Coffee. And chocolate. Teas of various sorts. Snacks – lots of snacks. We are all working hard. Long hours running 19+ days straight – we supply ourselves with the appropriate amounts of sugar and caffeine to keep us going.

Will Travel. Heck yeah! Shannon Point Marine Center in Anacortes, Washington –> SeaTac –> Anchorage –> Seward –> Prince William Sound –> Copper River plume in the eastern Gulf of AK –> Kodiak Island and 100 nautical miles south in the western Gulf of AK –> then back. In just 3 weeks!

I packed thousands of pounds of gear, supplies and equipment. Last but not least, I packed drawings from my boys. Ansel drew a boat and mermaid. Lev is still experimenting with his abstract phase.

All of the above adds up to a pretty exciting and interesting job as a member of Team Plankton. I enjoy working at sea, and my small boys think their mom has a super cool job.

P.S. Happy birthday, Jens. One of the drawbacks to life at sea. I wish I were there to celebrate!

My first research cruise

By Delphina, REU Student

Protective gear for loading day

I’ve been at sea before, but never for a long time and never while researching. I’ve been out on dive boats and cruise ships, but neither experience gave me a sense of what a research cruise would be like. I’d never been out for longer than a week and never particularly far from shore. I knew what projects we’d be working on and that we’d be working a lot, but everything else was a mystery. What is the schedule like? How are the rooms? How much does the boat rock? What is the lab space like?

My first surprise was just how much equipment it takes to run a lab. On land, you barely notice the closets of chemicals, drawers of gloves and tubing, and shelves of empty bottles.  But when you set up a lab from scratch, box by box, you realize how many supplies are needed to run the experiments. This is compounded by the need to bring extras and backups – there are no supply runs at sea – and by the need for organizers to keep supplies secure but accessible. As someone who stresses over remembering to pack enough t-shirts, I have no idea how someone manages to pack an entire lab without forgetting anything! The planning and organization involved in an operation like this is staggering, especially considering that we are only one of a handful of lab groups.

My next surprise was how high tech and comfortable the ship was. I hadn’t pictured ceilings lined with outlets, monitors in every room, an onboard crane, an elevator, and an RO water system. If you ignore the portholes, the labs feel almost like any other land-based lab; they have multiple sinks, fume hoods, freezers and enough space for all the scientists. In terms of commodities, I had expected to be roughing it a bit, imagining short showers and a bed reminiscent of my old dorm room. Instead, I was welcomed with plenty of hot water, a memory foam mattress, and the biggest towel I’ve ever seen. 

One thing I knew about, but hadn’t really considered, was how everything needs to be secure. In every room, there are tiny, ingenious modifications to keep things from flying around in bad weather. Drawers latch shut and tables have raised lips. In the mess, the coffee machines are bolted down and the placemats are nonslip. The deck and the lab benches are fitted with eyebolts so equipment can be tied down with ropes. And for the people there are handholds everywhere, even the shower.

DOC (Dissolved Organic Carbon) samples I collected and froze.

The science has so far been more what I expected. I’m ever more thankful to my lab classes for covering the basics, like opening a niskin bottle and vacuum filtering. But there are still a lot of new skills, like running a fluorometer and filtering size fractions. Staying organized is also a skill itself, keeping track of a large volume of samples and multiple experiments.

So far, a research cruise is a lot of work, especially since the rocking motion makes me sleepy. But contributing to research that’s been ongoing for decades feels amazing, like I’m part of something bigger than I realized. During our downtime, it’s fun to hear about everyone’s work, both the scientists and the crew.

Tomorrow we head to the plume and begin the experiment that’s the basis of my REU (Research Experiences for Undergraduates) project. Stay tuned for more information about this dilution experiment!

Gremlins on board – and we find the plume!

Satellite view of our region from two days ago (June 29th), showing the Copper River plume as a turquoise spiral at right. A cloud bank lurks offshore. Smoke from a wildfire on the Kenai Peninsula spirals into the atmosphere left of the cloud bank, and the silty waters of Turnagain Arm can be seen at upper left. NASA MODIS Terra image.

Wasn’t I just saying that it doesn’t do to get too attached to your daily agenda out here? Today the gremlins were out in force. Our morning started with a line wrapped around the ship’s propeller, a circumstance that arose during deployment of a sediment trap array (more on this in a later post). Needless to say this stopped science PDQ. After a tense hour or so, our incredibly able crew was able to get the line unwrapped by reversing the propeller slowly, while also launching the skiff to recover the rest of the array before it floated off into the sunrise. If you are getting the idea that oceanography depends as much on ships’ crews as it does on card-carrying oceanographers, you would be 100% correct!

Top of the CTD water sampling and instrument package, in the river plume waters 10 nautical miles offshore. The fine suspended rock particles, ground by glaciers, scatter white light and turn the normally blue-green coastal waters a brilliant turquoise.

On the positive side, We Found The Plume! At the inner end of our Middleton Island sampling line (a.k.a. the MID line), we encountered turquoise waters more reminiscent of a tropical lagoon than of subarctic seas. Glaciers grind rock into tiny particles (‘glacial flour’) which are carried into the Gulf of Alaska from glacier-fed rivers and provide an important source of the micronutrient iron (see post from June 24th). At 10 nautical miles offshore we were in water heavily influenced by the Copper River, with only a third of the salt content typical for the region. During our fine-scale survey, scheduled to start on Wednesday (but see top paragraph. . .), it looks like we’ll be mapping some highly complex and fascinating river plume features.

Team Phytoplankton completed yet more CTD sampling and experimental work. We found time to take a peek at some of the water samples we are collecting, and got a surprise – in waters 20 nautical miles offshore, there was a thriving community of tintinnid* ciliates. These single-celled organisms swim and feed using hair-like cilia at one end of the cell, and build a robust shell into which they can retract to escape predators. These particular tintinnids build their shells out of mineral grains found in the surrounding waters. We usually don’t see very many tintinnids in our study area, and are curious as to why they, and not the ‘typical’ ciliates, are so numerous here. These are the kinds of questions that get marine ecologists very excited – we’re an easily entertained bunch!

Tintinnid ciliates from the Copper River plume, captured today using a plankton net. The blue color is an artifact of the photography method. The hair-like structures visible at the ends of some of the cells are the cilia used for swimming and prey capture. Each cell is less than 50 micrometers (or 1/20th of a millimeter) in length. Sample and photo were taken by LTER project scientist Russ Hopcroft.

I have to go now – one of the ship’s cooks just came through the lab with a platter of warm chocolate chip cookies –

*”tintinnid” means “bell-like”. They are aptly named.

Everybody’s Doin’ It!

Evening on deck in Prince William Sound

Today was our first full day of science work, and it was a long and eventful one. We started off later than planned because Sikuliaq and her crew were called to assist a boat stranded on the remote southern coastline of Montague Island, at the entrance to Prince William Sound. The ship’s skiff was deployed late last night and some intrepid crew members were able to pull the stranded boat off the rocks. All in a day’s (or night’s) work on the remote coast of the Gulf of Alaska, and also a set-back for our work schedule. There is pretty much always some sort of set-back when working at sea, which promotes either high blood pressure or humility and a spirit of acceptance.

Much science was launched (hah!) today. The waters inside Prince William Sound were as flat as a millpond, helping immensely with all the training and new activities. Collectively, the science party and crew deployed and recovered a floating sediment trap array, did a variety of net tows and multiple CTD casts, sampled for trace metals using a special CTD and a surface-towed ‘iron fish’, and tested the Acrobat (towed instrument package) that we’ll be using to survey the Copper River plume.**

Hana adds nutrients to her mixotrophy experiment, working in one of the walk-in environmental chambers on board the ship to keep all the plankton in the bottles at sea surface temperatures (about 13°C).

Meanwhile, Team Plankton was crushing it. WWU graduate student Hana B. set up her first mixotrophy grazing experiment – a quick look through the microscope showed that Prince William Sound is teeming with photosynthetic dinoflagellates at the moment, so we have high hopes (see her blog post from June 27th).

Clay rummages for vials containing dark-acclimated plankton, ready to have their photosynthetic efficiencies checked – sort of like going in for your yearly physical –

WWU graduate student Clay M. got his first size-fractionated chlorophyll and photosynthetic efficiency data, while REU student Delphina W-P learned about sampling the CTD for dissolved organic carbon and chlorophyll, and ran her first chlorophyll analyses on the fluorometer. It’s safe to say that much was learned on many levels today!

And Team Plankton Super Tech Kerri F. holds it all together for us. Here she has a whole rack of samples filtering for analysis of particulate organic carbon content. While still smiling!

** We’ll write more about these methods in future posts but here’s the acronym of the day: CTD stands for Conductivity, Temperature, Depth and is the workhorse instrument package of any oceanographic expedition that is studying the water ‘column’ (as opposed to the bottom of the ocean).

Tying One On

The main lab, not quite ready for prime time
Buoys being craned on to Sikuliaq under smoky skies

Today was loading day, or in the local parlance, “mobilization”. We mobilized, alright. Some 50 or more pallets were craned onto R.V. Sikuliaq as she sat the the dock by Seward Marine Center, swathed in smoke from a forest fire in the region. Each of those pallets was loaded with many boxes, and each of those boxes was packed with gear that needed to be shlepped, sorted, tied down, set up, or otherwise dealt with. It’s always an exhausting day but at the same time it is exciting just to be STARTING, after all of the planning and anticipation.

As anyone who’s been seasick knows, ships move around, sometimes a lot. Every item that sits on a lab bench, counter, or table has to be tied down (‘secured’ – there is a different word for absolutely everything on a ship). This is the centrifuge we’ll use when we extract chlorophyll from phytoplankton to estimate the quantity present in the water, and today it was also the subject of a tutorial on bowlines and half-hitches.

Our great group of plankton researchers worked all day to move gear around through awkward spaces, set it up, and tie it down (oops – secure it!). We had our first meals on board, reconnected with friends and colleagues from prior cruises, made last-minute runs to the hardware store and Safeway (dark chocolate!), and discovered things we’d forgotten and will now have to do without. Tomorrow after breakfast we sail (yes, sail, even though the ship is diesel-electric), get our safety briefing, and occupy our first sampling stations. Now for the shower and my bunk after a VERY long day. . .

Members of Team Phytoplankton (grad student Hana Busse and REU student Delphina Walker-Phelan) get their plumbing groove on while setting up our deck incubators
View of the back deck (‘fantail’) while refueling – a lot of the chaos has already been tamed (note large pile of empty pallets at right). Team Zooplankton, at the stern, is assembling the frame of a large townet for capturing jellies.

It’s a plant – it’s an animal – hey, it’s both!

Post by WWU Graduate Student Hana Busse

In addition to our science crew and gear, live cultures of cryptophytes and cyanobacterium are boarding the plane from SeaTac to Anchorage today. These microscopic organisms are taking this journey to help us answer questions about the role of mixotrophy in subarctic ecosystems.

Laboratory cultures of marine cyanobacteria (genus Synechococcus), the smallest phytoplankton in our study region. Photo courtesy Woods Hole Oceanographic Institution.
Small photosynthetic dinoflagellate (chloroplasts fluorescing red) with ingested cyanobacterial cell (yellow)

Mixotrophy, the ability to gain energy through a combination of feeding and photosynthesis, has been observed globally and is employed by a diverse population of single-celled organisms. This flexibility in trophic strategy is believed to help organisms survive in times of low light, when photosynthesis is limited, or during periods of prey scarcity. Despite the fact that mixotrophy is ubiquitous throughout our oceans and has evolved within many planktonic lineages, the ecological role of this phenomenon is largely unexplored.

In the Northern Gulf of Alaska (NGA), where large fluctuations in environmental conditions are common, we think mixotrophy may be an especially important strategy for survival. To better understand the link between environmental conditions and trophic strategy, we will be using our cultured cryptophytes and cyanobacterium as prey for naturally occurring mixotrophic dinoflagellates under gradients of light availability, prey concentration, and nutrient concentration.

These experiments will help us understand how mixotrophy acts to buffer marine communities in the face of environmental fluctuations, giving these beautiful ‘jack-of’all-trades’ species access to food when light or nutrients are scarce, and photosynthetic production when prey are scarce. This buffering capacity is a particularly important topic as climate change increases daily, annual and interannual variability in the Gulf of Alaska.

The large dinoflagellate Ceratium (chloroplasts fluorescing red) with ingested cryptophyes (yellow-orange inclusion).